Mitooma District is bordered by Bushenyi District to the north, Sheema District to the east, Ntungamo District to the south, and Rukungiri District to the west. Mitooma, where the district headquarters are located is located approximately 25 kilometres (16 mi), by road, southwest of Bushenyi, the nearest large town. This location lies approximately 85 kilometres (53 mi), by road, west of Mbarara, the largest city in Ankole sub-region. The coordinates of the district are:00 36S, 30 00E.
The district was created by Act of Parliament and became functional on 1 July 2010. Prior to then, the district used to be Ruhinda County in Bushenyi District. Mitooma district is part of Ankole sub-region. The sub-region, which is coterminous with the Ankole Kingdom, comprises the following districts: 1. Buhweju District 2. Bushenyi District 3. Ibanda District 4. Isingiro District 5. Kiruhura District 6. Mitooma District 7. Ntungamo District 8. Rubirizi District and 9. Sheema District. The sub-region was home to an estimated 2.2 million people as of 2002, according to the national census conducted that year.